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Services ~ Cytotoxicity Testing

Our testing is designed to determine the cytotoxic effects of leachables from a device/material. Our testing is in compliance with ISO 10993-5 standard. We grow the specific cell line to subconfluency before the test device is placed directly on the adhered cells. The cells and test article are incubated together at 37 degrees for 24 hours the samples are examined under microscope.

The microplate containing the exposed cell cultures are incubated with a cell dye which can be used to quickly measure the number of live cells in each of the wells. The cell dye provides sensitive colorimetric assays for the determination of the number of viable cells in cell proliferation and cytotoxicity assays. The amount of the dye generated, which is directly proportional to the number of living cells, is determined by measuring the absorbance at 450nm.

The absorbance is compared to the untreated controls to determine the percentage of surviving cells. This is used to determine the cytotoxicity of the tested compounds/devices.

Figure 1. Negative controls used to create Standard curve of viable cell density vs. absorbance
Figure 2. Cell viability post 24 hr treatement of devices A, C and D.
Figure 3. % kill post 24 hr treatement of compounds devices A, C and D.

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